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Tris, commonly used as a buffer in biochemistry and molecular biology, stabilizes the pH of solutions in electrophoresis or other biological applications. It is also an enzyme inhibitor for use in the biopharmaceutical industry and is commonly utilized in electro chromatography, UV analysis and HPLC applications.
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NH2C(CH2OH)3 ^ F.W. 121.14 g/mol ^ CAS# 77-86-1 |
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Test |
Specifications |
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Bio Ultra |
Bio Synthesized |
Bio Industrial |
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TRS-13 |
TRS-12 |
TRS-11 |
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Manufactured in the |
Pass |
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Appearance and Color |
White/Crystals |
White/Crystals |
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Insoluble Matter |
0.005% max. |
0.005% max. |
0.005% max. |
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Assay, Dried Basis |
99.9% min. |
99.5% min. |
99.0% min. |
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Identification (IR) |
Match Reference |
Match Reference |
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Karl Fisher Water |
0.5% max. |
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Melting Range/C |
168-172 |
168-172 |
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Absorbance (40%) |
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0.05 max. @ 400 |
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0.2 max. @ 290 |
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0.2 max @ 290 |
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0.01 max. @ 400 |
0.02 max. @ 400 |
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Absorbance (1M) |
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0.06 max. @ 280 |
0.08 max. @ 280 |
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0.06 max. @ 260 |
0.08 max. @ 260 |
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Loss on Drying |
1.0% max |
1.0% max. |
1.0% max. |
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Sodium (Na) |
0.005% max. |
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Potassium (K) |
0.005% max. |
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Arsenic (As) |
0.0001% max. |
0.0001% max. |
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Calcium (Ca) |
0.0001% max. |
0.0001% max. |
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Copper (Cu) |
0.0001% max. |
0.0001% max. |
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Iron (Fe) |
0.0001% max. |
0.0001% max. |
0.0005% max. |
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Magnesium (Mg) |
0.0001% max. |
0.0001% max. |
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Lead (Pb) |
0.0001% max. |
0.0001% max. |
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USP Heavy Metals |
0.0005% max. |
0.0005% max. |
0.0005% max. |
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pH 5% Solution |
10.0-11.5 |
10.0-11.5 |
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Residue On Ignition |
0.1% max. |
0.1% max. |
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RNase |
None Detected |
None Detected |
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DNase |
None Detected |
None Detected |
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Protease |
None Detected |
None Detected |
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Beilstein Beil. 4,IV,1903
Merck Merck 13,9842
"Dissociation constants and related thermodynamic quantities of the protonated acid form of tris-(hydroxymethyl)-aminomethane in mixtures of 2-methoxyethanol and water at different temperatures". J. Electroanal. Chem. 162 (1-2): 285–305. doi:10.1016/S0022-0728(84)80171-0.
Gomori, G., Preparation of Buffers for Use in Enzyme Studies. Methods Enzymology., 1, 138-146 (1955).
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| Bio Industrial (Good) Quality tested from approved suppliers of BBS for Industry-Standard use. |
| Bio Synthesized (Better) Manufactured in a quality system based facility offering traceability and consistent supply. |
| Bio Ultra (Best) Manufactured at BBS to meet the highest quality and regulatory standards. |
