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Hepes Hemisodium is a zwitterionic buffer that is used in cell culture, electrophoresis and molecular biology applications. HEPES Hemisodium is useful in biochemical reaction because it is chemically stable and has very little effects within a reaction. It is considered an ideal buffer because of its high solubility factor and because its pKa is between 6.0 and 8.0.
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(N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid), hemisodium salt) |
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C8H17.5N2Na0.5O4S ^ F.W. 249.3 g/mol ^ CAS# 103404-87-1 |
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Test |
Specifications |
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Bio Synthesized |
Bio Industrial |
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HPN-12 |
HPN-11 |
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Appearance and Color |
White/Crystals |
White/Crystals |
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Assay, dried basis |
99.5% min. |
99.0% min. |
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Identification (IR) |
Match Reference |
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Absorbance |
0.03 max. @ 280 |
0.03 max. @ 280 |
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0.04 max. @ 260 |
0.04 max. @ 260 |
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Loss on Drying |
3.0% max. |
3.0% max. |
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Arsenic (As) |
0.0001% max. |
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Copper (Cu) |
0.0001% max. |
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Iron (Fe) |
0.0001% max. |
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Lead (Pb) |
0.0001% max. |
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pH (1%) |
7.2-7.6 |
7.2-7.6 |
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pKa |
7.4-7.6 |
7.4-7.6 |
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RNase |
None Detected |
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DNase |
None Detected |
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Protease |
None Detected |
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| Bio Industrial (Good) Quality tested from approved suppliers of BBS for Industry-Standard use. |
| Bio Synthesized (Better) Manufactured in a quality system based facility offering traceability and consistent supply. |
| Bio Ultra (Best) Manufactured at BBS to meet the highest quality and regulatory standards. |
