Tris Hydrochloride, CAS# 1185-53-1

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Tris Hydrochloride

Molecular Fomula: C₄H₁₁NO₃ClH
F.W.: 157.6
CAS#: 1185-53-1
pH: 4.0 - 5.0


Bio Industrial	Bio Synthesized	Bio Ultra

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Description:
Tris HCl is used as a stabilizing buffer in biological applications such as electro chromatography, UV analysis and HPLC. Tris HCl is utilized to adjust pH ranges for gels used in electrophoresis applications.

Price Comparison Specifications Product Links

x 100g size

	BioBuffer Solutions	Sigma	Amresco	Research Organics	Calbiochem
Grade	Bio Ultra	Trizma® base BioUltra**		Research Organics	Molecular Biology
Product Code	THL-13	93363		3098T	648317
Assay	99.5% min	≥99.0%		≥99.5%	≥99%
Price	$19.00	$109.50		$31.50	$49.00

Grade	Bio Synthesized	Trizma® base**
Product Code	THL-12	T3253
Assay	99.0-103.0%	≥99.0%
Price	$15.50	$38.50

Grade	Bio Industrial
Product Code	THL-11
Assay	99.0% min.
Price	$12.00

**Trizma® is a registered trademark of Biotechnology LP and Sigma-Aldrich Co.

x 250g size

	BioBuffer Solutions	Sigma	Amresco	Research Organics	Calbiochem
Grade	Bio Ultra	Trizma® base BioXtra**			ULTROL®*
Product Code	THL-13	T6666			648313
Assay	99.5% min.	≥99.0%			≥99%
Price	$38.00	$102.90			$69.00

Grade	Bio Synthesized	Trizma® base**
Product Code	THL-12	T3253
Assay	99.0-103.0%	≥99.0%
Price	$31.00	$54.90

Grade	Bio Industrial
Product Code	THL-11
Assay	99.0% min.
Price	$24.00

*ULTROL® is a registered trademark of Calbiochem and EMD Chemicals Inc.
**Trizma® is a registered trademark of Biotechnology LP and Sigma-Aldrich Co.

x 500g size

	BioBuffer Solutions	Sigma	Amresco	Research Organics	Calbiochem
Grade	Bio Ultra	Trizma® base BioUltra**	Ultra Pure	Research Organics	ULTROL®*
Product Code	THL-13	93363	0234	3098T	648313
Assay	99.5% min.	≥99.0%	99.5%	≥99.5%	≥99%
Price	$57.00	$301.00	$99.00	$79.70	$109.00

Grade	Bio Synthesized	Trizma® base**
Product Code	THL-12	T3253
Assay	99.0-103.0%	≥99.0%
Price	$46.50	$103.00

Grade	Bio Industrial
Product Code	THL-11
Assay	99.0% min.
Price	$36.00

*ULTROL® is a registered trademark of Calbiochem and EMD Chemicals Inc.
**Trizma® is a registered trademark of Biotechnology LP and Sigma-Aldrich Co.

Tris Hydrochloride

C₄H11NO₃ClH ^ F.W. 157.6 g/mol ^ CAS# 1185-53-1

Test	Specifications

	Bio Ultra	Bio Synthesized	Bio Industrial
	THL-13	THL-12	THL-11

Manufactured in the USA	Pass
Appearance and Color	White/Crystals	White/Crystals	White/Crystals
Insoluble Matter	0.001% max.
Assay, Dried Basis	99.5% min.	99.0-103.0%	99.0% min.
Identification (IR)	Match Reference	Match Reference
Solubility	Clear
Melting Range/C	150-153	147-153
	0.01 max. @ 400	0.01 max. @ 400	0.01 max. @ 400
Absorbance	0.06 max. @ 280	0.06 max. @ 280
	0.06 max. @ 260
Loss on Drying	0.5% max.	0.5% max.	1.0% max.
Arsenic (As)	0.0001% max.	0.0001% max.
Calcium (Ca)	0.0001% max.	0.0001% max.
Copper (Cu)	0.0001% max.	0.0001% max.
Iron (Fe)	0.0001% max.	0.0001% max.
Magnesium (Mg)	0.0001% max.	0.0001% max.
Lead (Pb)	0.0001% max.	0.0001% max.
Heavy Metals	0.0002% max.	0.0005% max.	0.0005% max.
pH (0.5M)	4.0-5.0	3.5-5.0	3.5-5.0
pK_a	8.0-8.4
Residue On Ignition	0.1% max.
RNase	None Detected	None Detected
DNase	None Detected	None Detected
Protease	None Detected	None Detected

Tris HCl

Use of pH 9.5 Tris-HCl buffer containing 5% urea for antigen retrieval immunohistochemistry.

Product	Molecular	CAS #	Details
Tris Hydrochloride	C₄H₁₁NO₃ClH	1185-53-1
PIPES, Free Acid	C₈H₁₈N₂O₆S₂	5625-37-6
MES, Monohydrate	C₆H₁₃NO₄S H₂O	4432-31-9

Bio Industrial (Good)
Quality tested from approved suppliers of BBS for Industry-Standard use.

Bio Synthesized (Better)
Manufactured in a quality system based facility offering traceability and consistent supply.

Bio Ultra (Best)
Manufactured at BBS to meet the highest quality and regulatory standards.

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