HEPES Free Acid Solution CAS# 7365-45-9

HEPES Free Acid Solution

Molecular Fomula: C₈H₁₈N₂O₄S
F.W.: 238.3
CAS#: 7365-45-9

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Description:
HEPES is a Zwitterionic Buffer used to maintain pH of media used in cell cultures. It is one of Good's twelve buffers that has a pKa value similar to its pH value making it an ideal buffer for pH maintenance. It has only one restriction, it interferes with the Folin protein assay. This buffer can form radicals so it is not suitable for redox studies.

Specifications Product Links

HEPES Free Acid Solution

(N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid)
C₈H₁₈N₂O₄S ^ F.W. 238.3 g/mol ^ CAS# 7365-45-9

TEST		SPECIFICATIONS

		1.0 Molar HPLA	0.1 Molar HPLB

Appearance and Color		Clear/Colorless	Clear/Colorless
Solutions Test		Pass	Pass
Concentration		0.99 - 1.01M	0.09 - 0.11M
pH		5.0 - 6.5	5.0 - 6.5
pKa		7.45 - 7.65	7.45 - 7.65
Conductivity		Report	Report
Trace Metals	Sodium (Na)	0.005% max.	0.005% max.
	Potassium (K)	0.005% max.	0.005% max.
	Arsenic (As)	0.00005% max.	0.00005% max.
	Copper (Cu)	0.0001% max.	0.0001% max.
	Iron (Fe)	0.0001% max.	0.0001% max.
	Magnesium (Mg)	0.0001% max.	0.0001% max.
	Lead (Pb)	0.0001% max.	0.0001% max.
Enzymes	RNase	None Detected	None Detected
	DNase	None Detected	None Detected
	Protease	None Detected	None Detected

Product	Molecular	CAS #	Details
Bicine	C₆H₁₃NO₄	150-25-4
MES, Monohydrate Solution	C₆H₁₃NO₄S•H₂0	4432-31-9
Ammonium Formate	NH₄HCO₂	540-69-2

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