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Hepes is a Zwitterionic Buffer used to maintain pH of media used in cell cultures. It is one of Good's twelve buffers that has a pKa value similar to its pH value making it an ideal buffer for pH maintenance. It has only one restriction, it interferes with the Folin protein assay. This buffer can form radicals so it is not suitable for redox studies.
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(N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid) |
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C8H18N2O4S ^ F.W. 238.3 g/mol ^ CAS# 7365-45-9 |
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Test |
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Specifications |
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Bio Ultra |
Bio Synthesized |
Bio Industrial |
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HEP-13 |
HEP-12 |
HEP-11 |
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Manufactured in the |
Pass |
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Appearance and Color |
White/Powder |
White/Powder |
White/Powder |
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Insoluble Matter |
0.01% max. |
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Assay |
99.9% max. |
99.5% min. |
99.0% min. |
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Identification (IR) |
Match Reference |
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Solubility |
Clear/Complete |
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Chloride |
0.005% max. |
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Sulfate |
0.005% max. |
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0.05 max. @ 280 |
0.05 max. @ 280 |
0.05 max. @ 280 |
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Absorbance |
0.05 max. @ 260 |
0.05 max. @ 260 |
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0.08 max. @ 250 |
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Loss on Drying |
0.5% max. |
0.5% max. |
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Sodium (Na) |
0.005% max. |
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Potassium (K) |
0.005% max. |
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Arsenic (As) |
0.00005% max. |
0.00005% max. |
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Copper (Cu) |
0.0001% max. |
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Iron (Fe) |
0.0001% max. |
0.0005% max. |
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Magnesium (Mg) |
0.0001% max. |
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Lead (Pb) |
0.0001% max. |
0.0005% max. |
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Heavy Metals |
0.0001% max. |
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pH 5% Solution |
5.0-6.5 |
5.0-6.5 |
5.0-6.5 |
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pKa |
7.45-7.65 |
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Residue On Ignition |
0.1% max. |
0.2% max. |
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RNase |
None Detected |
None Detected |
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DNase |
None Detected |
None Detected |
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Protease |
None Detected |
None Detected |
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| Bio Industrial (Good) Quality tested from approved suppliers of BBS for Industry-Standard use. |
| Bio Synthesized (Better) Manufactured in a quality system based facility offering traceability and consistent supply. |
| Bio Ultra (Best) Manufactured at BBS to meet the highest quality and regulatory standards. |
