Extensive Biological Buffers Product Line – HEPES, PIPES, MOPS Sodium, GMP Buffers
BioBuffer Solutions offers a complete line of Biological Buffers which include: PIPES, HEPES, HEPES Sodium, Pipes Sodium, MES Sodium, MOPS Sodium, Tris Hydrochloride, Guanidine Hydrochloride, MOPS Buffer, GMP Buffers, and TRIS also known as Trizma®.
PIPES, MES Sodium & HEPES – Good’s Buffers
PIPES, MES and HEPES are among the twelve biological buffers selected by Norman Good, otherwise known as Good’s Buffers.
PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), a frequently used buffering agent in biochemistry. PIPES Sodium is 1,4-Piperazinediethanesulfonic acid sodium salt, Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt with a useful pH range of 6.1 - 7.5.
MES Sodium is 2-(N-Morpholino)ethanesulfonic acid sodium salt and 4-Morpholineethanesulfonic acid sodium salt. It has a useful pH range of 5.5 - 6.7.
HEPES is the common name for (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ), a Zwitterionic organic chemical buffering agent. HEPES is widely used in cell culture because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. HEPES Sodium is 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt and N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt. It has a useful pH range of 6.8 - 8.2.
More Biological Buffers
MOPS Sodium is the buffer’s popular name for the compound 3-(N-morpholino) propanesulfonic acid. MOPS Sodium is an excellent buffer for many biological systems at near-neutral pH. MOPS Buffer is most frequently used as a buffering agent in biology and biochemistry.
Tris Hydrochloride or Tromethamine HCl is used as an intermediate for the preparation of surface active agents, vulcanization accelerators and pharmaceuticals. Tris Hydrochloride has a pH range of 3.5 - 5.0. Tris Hydrochloride is extensively used in biochemistry and molecular biology. In biochemistry, Tris Hydrochloride is widely used as a component of buffer solutions.
Guanidine Hydrochloride (Guanidine HCI) is a crystalline compound of strong alkalinity formed by the oxidation of guanine.
TRIS also known as Trizma® is a biological buffer solution commonly used in molecular biology. Tris(hydroxymethyl)aminomethane. Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.Trizma HCl is Tris Hydrochloride
Biological Buffers Composed of Bulk, Laboratory Reagents & Liquids
BioBuffer Solutions’ product lines offer our customers the flexibility of ordering our products based on their individual application needs.
Biological Buffers’ Bulk Product Line
The bulk product line is intended to supply the biological buffer market with convenient packaging at the best value seen in the biopharmaceutical industry. This bulk product line is specifically designed with the understanding of what is important for your processes and ensures that the highest level of quality is provided.
Biological Buffers’ Lab Reagent Product Line
The lab reagent product line was designed to meet biopharm and biotech laboratory needs in terms of sizes, pricing and delivery. We can guarantee to offer the highest quality of product at a better value. Through our grade selection you can select the product that is right for you. To make things even more convenient we have established a range of package sizes that would be most suitable for your laboratory applications.
Biological Buffers’ Liquid Product Line
The liquid product line was created to ensure that all your custom solutions could be made directly from your manufacturer. We can provide custom made solutions in a variety of sizes for your convenience. We can also provide custom blends of products to meet your specifications while maintaining the best value on the market.
Solubility, pH and pKa Chart of BioBuffer Solutions' Products |
||||
| Product | Molecular weight g/mol |
pH Range @ 20C |
avg pKa @20C |
Solubility g/mL @25C |
| Hepes, free acid | 238.3 | 5.6-6.5 | 7.5 | 8.55 |
| Hepes Na | 260.3 | 6.8-8.2 | 7.5 | 0.99 |
| Hepes Hemisodium | 249.3 | 7.2-7.3 | 7.5 | N/A |
| Mes, Monohydrate | 213.25 | 2.5-4.5 | 6.1 | 0.31 |
| Mes Na | 217.22 | 5.5-6.5 | 6.1 | 0.59 |
| Mops, free acid | 209.26 | 3.0-4.5 | 7.2 | 0.98 |
| Mops Na | 231.2 | 10.0-11.0 | 7.2 | 1.01 |
| Pipes, free acid | 302.37 | 6.0-7.5 | 6.8 | N/A |
| Pipes Na | 335.82 | 6.0-7.5 | 6.8 | 0.9 |
| Tris | 121.51 | 10.0-11.5 | 8 | 0.65 |
| Tris Hydrochloride | 157.6 | 4.0-5.0 | 8.2 | 1.01 |
| Guanidine Hydrochloride | 99.53 | 4.5-6.0 | N/A | 2.15 |
| Guanidine Thiocyanate | 118.16 | 5.0-7.0 | N/A | N/A |
| Urea | 60.06 | 7.5-9.5 | N/A | 1.2 |
| Solubility, pH and pKa results are based on analytical testing results. | ||||
